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Course In :
NMR Spectroscopy (4p), a four week course for fourth year students of the
Molecular Biotechnology programme
The goal of this 7-week course in NMR Spectroscopy is to make the students
familiar with the basic physics of NMR, the basics behind one- and
multi-dimensional NMR as well as how NMR techniques can be used to determine
the structure and conformation of biological macromolecules.
During the course we use both lectures and practical sessions to achieve these
goals. In the lab-sessions we use our 600 MHz and our two 500 MHz NMR machines
to acquire data and then we spend quite a lot of time on our Silicon Graphics
workstations to analyze our NMR data.
We will also have 'tutorials' which would be something similar to a
In the tutorial sessions you will be given materials for self-studies of
'problem-solving' type. You will for instance be given NMR spectra for assignment,
there will also be problems of solving second-order spectra, etc.
One of our teachers will be on hand to help you if necessary.
The lab-sessions include analysis of different kinds of 1D- and 2D-NMR spectra:
How assignment can be simplified by combining DQF-COSY with NOESY spectra,
how to extract H-H distance information from NOESY spectra, how to estimate
torsional angles from experimental coupling constants etc.
At the end of the course we build a model of a DNA duplex using data derived from
NMR spectroscopy (mainly NOE distance information).
In some lab sessions, we will not use spectrometers or computers at all: Instead
we will work with paper (NMR spectra) and pencil only. We are obliged to do
this since there are not enough spectrometers and computers available.
Students attending our NMR course should have passed the following courses:
Organic Chemistry with Analytical Techniques, Methods in Biochemistry,
Biomolecular Structure, Molecular and Statistical Mechanics, and
Some knowledge of quantum mechanics is also quite useful.
- The course will be given to fourth-year students following the
'Molecular Biotechnology' program.
Others who are interested can contact
Prof. Jyoti Chattopadhyaya or
- The course will be given next time in April-May 2000
- The length of the course is approx. 7 weeks (Apr 5 - May 20, ) with 16 working days.
- 40 hrs of lectures.
- 39 hrs of practical work.
- 14 hrs of tutorials.
- 93 hrs total scheduled time.
- ~130 hrs estimated total working time.
- Provide a basic knowledge for structure determination of biopolymers
in aqueous solution by NMR spectroscopy
- Familiarize the student with the structural (eg. dihedral and distance)
information that can be used to understand
- dynamic aspects of biomolecular structure
- function of biomolecules
- interactions between biomolecules.
- Organic chemistry with analytical techniques
- Biochemical Methods
- Biomolecular structure
- Molecular and statistical mechanics
- Biophysical chemistry.
- Basic principles of NMR
- Function of the spectrometer and basic experimental procedures
- Chemical shifts
- Coupling constants
- Determination of torsional angles via Karplus equations
- Stereo chemistry
- Double resonance and relaxation processes
- The nuclear Overhauser effect (NOE)
- Chemical exchange processes and their use for measuring kinetic and thermodynamic properties.
- Basic principles for 2D NMR experiments (homo and heteronuclear shift
correlations, NOESY and ROESY).
- Practical work
- Analysis of chemical shifts in 1D NMR spectra of proton and carbon
- Measurement and analysis of 1H-1H, 1H-13C,
1H-31P coupling constants in 1D and 2D NMR spectra
- Measurement of 1H-1H distances from 1D and 2D NOE experiments for the
determination of the stereochemistry of a molecule as well as for the
assessment of dynamical processes of a molecule
- Determination of structure and conformation of simple biomolecules by measuring
both coupling constants (torsional angles) and NOE intensities (distances)
Dep't of Bioorg. Chem.
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